Journal: Nanomaterials

Article Title: Impact of Carbon Nano-Onions on Hydra vulgaris as a Model Organism for Nanoecotoxicology

doi: 10.3390/nano5031331

Figure Lengend Snippet: Raman spectra of p-CNO (black line), benz-CNO (red line), py-CNO (blue line) and py+-CNO (green line).

Article Snippet: Raman spectra were measured on a 800 UV LabRam Raman microscope (Horiba Jobin Yvon, Longjumeau, France).

Techniques:

Journal: Developmental biology

Article Title: Strains matter: Success of murine in vitro spermatogenesis is dependent on genetic background.

doi: 10.1016/j.ydbio.2019.08.007

Figure Lengend Snippet: Fig. 4. Immunohistochemical identification of the steroidogenic function of Leydig cells by expression of CYP17A1 enzyme (brown) in mice testic- ular fragments sections throughout organ culture. A. C57BL/6 J (n ¼ 3) and B. B6D2F2 (n ¼ 4) mouse donors. Insets show isotype IgG negative controls. For both strains, CYP17A1 was expressed by Leydig cells before cultured and maintained during the entire culture period. Arrow-head: CYP17A1-positive Leydig cell. Scale bars represent 20 μm. Pictures were taken using an Olympus BX41 bright-field microscope with an Olympus DP20 color camera.

Article Snippet: Sections were subsequently incubated with 1:1000 rabbit polyclonal LIN28 (ab46020, Abcam, UK); 1:100mousemonoclonal AR (sc-7305, Santa Cruz Biotechnology, USA) or 1:800 rabbit polyclonal CYP17A1 (ab80206, Abcam, UK) primary antibody overnight at 4 C and, after washing, followed by 1 h incubation with goat-anti Mouse/Rabbit poly-HRP secondary antibody (DPVO110HRP, Immunologic, the Netherlands).

Techniques: Immunohistochemical staining, Expressing, Organ Culture, Cell Culture, Microscopy